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Quintessence Int 43 (2012), No. 8     26. July 2012
Quintessence Int 43 (2012), No. 8  (26.07.2012)

Page 703-707, PubMed:23034423


In vitro microbiologic evaluation of PTFE and cotton as spacer materials
Paranjpe, Avina / Jain, Sumita / Alibhai, Karim Z. / Wadhwani, Chandur P. / Darveau, Richard P. / Johnson, James D.
Objective: To microbiologically evaluate the efficacy of cotton and polytetrafluoroethylene (PTFE) tape used as spacer materials.
Method and Materials: Twenty-six extracted human molars were restored using either cotton or PTFE tape as spacers under a standardized provisional restorative material (Cavit). The teeth were incubated for 7 days in a culture of Streptococcus gordonii or in liquid media alone. The spacers were removed and tested for bacterial contamination. The access cavities were also evaluated for bacterial contamination.
Results: Nine of 10 teeth with cotton spacers and one of 10 teeth with PTFE spacers were positive for S gordonii growth. The nine teeth in the cotton group also showed contamination of the access cavities.
Conclusion: Even under optimal conditions, cotton spacers may cause leakage into the access cavities. Cotton fibers may serve as a route for bacterial contamination of the access cavities and root canal space. In contrast, PTFE tape did not provide an avenue for bacterial contamination.

Keywords: cotton, endodontics, PTFE, spacer