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Quintessence International
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Quintessence Int 22 (1991), No. 4     1. Apr. 1991
Quintessence Int 22 (1991), No. 4  (01.04.1991)

Page 277-283


Burnished versus nonburnished application of citric acid to human diseased root surfaces: the effect of time and method of application
Codelli / Fry / Davis
Citric acid (pH = 1) was applied passively or by vigorous burnishing for 3 or 5 minutes to diseased human root surfaces following root planing in vitro. Control surfaces were similarly treated with saline only. Teeth were fixed in glutaraldehyde, dehydrated, freeze-dried, and sputter coated with gold for scanning electron microscopic viewing. Results suggested that optimal exposure of collagen and emineralization occurred with the burnishing application of citric acid for 3 minutes. Although somewhat similar results were found with passive application for 5 minutes, the result of the burnishing application for 5 minutes was that the root was overdemineralized or that the exposed collagen was completely obscured by an amorphous layer that was suggestive of protein denaturization.