We are using cookies to implement functions like login, shopping cart or language selection for this website. Furthermore we use Google Analytics to create anonymized statistical reports of the usage which creates Cookies too. You will find more information in our privacy policy.
OK, I agree I do not want Google Analytics-Cookies
Quintessence International
Login:
username:

password:

Plattform:

Forgotten password?

Registration

Quintessence Int 29 (1998), No. 9     1. Sep. 1998
Quintessence Int 29 (1998), No. 9  (01.09.1998)

Page 585-593


In vitro evaluation of complex carbohydrate denture adhesive formulations
Tello / Ford / Iacopino
Objective: Acemannan, a complex mannose carbohydrate dervied from the aloe vera plant, has an inherent stickiness/viscosity. Prototype Acemannan denture adhesive for mulations were evaluated for pH changes, cytotoxicity to human gingival fibroblasts and adhesive strength in both dry and wet conditions. Method and materials: The denture adhesive formulations consisted of five combinations of Acemannan with varying concentrations of preservtives and two other formulations without preservatives. The pH of each formulation was measured over 24 hours. Assessment of cytotoxicity was accomplished using the in vitro, tetrazolium-based colorimetri c assay on cultures of human gingival fibroblasts after exposure to the adhesive formulations for up to 24 hours. The adhesive strength was evaluated with a universal testing machine under intial dry conditions and after immersion in a constant-temperature water bath for up to 20 minutes. Results: Formulations 1 and 2 acheived and maintained pH values above 6.0 (the critical pH for hydroxyapatite dissolution) approximately 6 hours into the study. None of the prototypes demonstrated an initial pH above the critical pH. Formulations 1, 2, 3, and 5 exhibited significant cytotoxicity to human gingival fibroblasts over 24 hours. Formulations 4, 20:1, and 150:1 demonstrated minimal cytotoxicity. Formulation 1 exhibited the poorest adhesive strength, while the most viscous formulation (prototype 150:1) was by far the best performer. Generally, adhesive bond strengths for all prototypes were quite high and relatively stable over time in a wet environment. Conclusion: To achieve the ideal adhesive in terms of strength, pH, and cytotoxicity. Acemannan formulation 150:1 should be adjusted to contain the preservative concentration of formulation 4 and have an initial pH value of 6.0 or higher.