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Quintessence International
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Quintessence Int 39 (2008), No. 2     8. Jan. 2008
Quintessence Int 39 (2008), No. 2  (08.01.2008)

Page 159-163, PubMed:18560654


An evaluation of bacterial contamination of barriers used in periapical tissue regeneration: Part 1-Bacterial adherence
Sharma, Priya / Mickel, André K. / Chogle, Sami / Sharma, Prem Nath / Han, Yiping W. / Jones, Jefferson J.
Objective: To compare the adherence of Prevotella melaninogenica and Enterococcus faecalis to 3 guided tissue regeneration membranes: Atrisorb, Lambone, and OsseoQuest. It was hypothesized that OsseoQuest would show increased bacterial adherence compared to Lambone and Atrisorb.
Method and Materials: The barriers were suspended in trypticase soy broth containing an inoculum of either P melaninogenica or E faecalis. The samples were incubated under appropriate conditions for 6, 24, and 48 hours. Following incubation, each membrane was mixed in fresh media in a vortex machine to dislodge adherent bacteria. The vortexed media was quantitatively assessed using serial dilutions for viable cell count.
Results: E faecalis exhibited higher adherence compared to P melaninogenica with time. Of the membranes tested, Lambone displayed the least bacterial adherence.
Conclusion: An analysis of the results indicated that bacterial adherence was time-dependent for all membranes. Membrane structure, chemical configuration, hydrophobicity, and bacterial cell surface structure were suggested as factors contributing to variance in bacterial adherence.

Keywords: bacteria, barrier membrane, periapical surgery